Parvin Shariati Gazgazareh; ALI AKBAR MASOUDI; Rasoul Vaez Torshizi; Alireza Ehsani; Zaynab Mousavian
Volume 22, Issue 2 , June 2020, , Pages 199-209
Abstract
The aim of this study was the investigation of gene expression profile in Shal sheep ovarian tissue using RNA sequencing data. For this purpose, the ovaries of five Shal sheep were isolated after estrous synchronization and their RNA was sequenced using Illumina Hiseq 4000 technology. On average, ...
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The aim of this study was the investigation of gene expression profile in Shal sheep ovarian tissue using RNA sequencing data. For this purpose, the ovaries of five Shal sheep were isolated after estrous synchronization and their RNA was sequenced using Illumina Hiseq 4000 technology. On average, the data obtained from the sequencing consisted of 26638311 read pairs with 81.08 unique mapping rate. The results of bioinformatic analyzes revealed the expression of 21085 genes in Shal sheep ovarian tissue, of which 15078 genes had expression mean above 10. Gene ontology analysis revealed the significant enrichment of 162 GO terms including 41 biological processes, 46 molecular functions and 75 cellular components. KEGG pathway analysis also identified 149 significant pathways (P <0.05), most important of which were estrogen signaling pathway, TGF-beta signaling pathway and oocyte meiosis. Investigating the expression of major genes for twining and reproduction, showed a high expression for INHA, INHBA and BMPR1B, so that INHA, an important paracrine factor in ovarian follicles, was one of the 10 genes with the highest expression. Also, FSHR, ESR1 and ESR2 showed medium expression and GDF9, BMP15 and PRLR showed low expression in the samples. For the first time, in this study the ovarian tissue transcriptome of Shal ewes was comprehensively studied using RNA-Seq technology and this study can provide a useful genetic basis for a better understanding of the genes and processes involved in the Shal sheep reproduction.
Afrooz Kamali Sangani; Ali Akbar Masoudi; Rasoul Vaez-Torshizi; Morteza Sharifi nia; Alireza Eyvazi; Majid Farahi; Behzad Rajabi Marand
Volume 18, Issue 3 , October 2016, , Pages 583-591
Abstract
This study was conducted to rank the broiler farms based on buildings, installations and equipment and its effect on production factors. To determine the contribution of each factor in ranking of farms, a multi-criteriadecision analysis (SAW) was used. Data of 108 broilers were recorded in specific forms ...
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This study was conducted to rank the broiler farms based on buildings, installations and equipment and its effect on production factors. To determine the contribution of each factor in ranking of farms, a multi-criteriadecision analysis (SAW) was used. Data of 108 broilers were recorded in specific forms and the amount of investment per broiler was calculated for individual factors. To calculate the adjusted weights of parameters, 20 experts were consulted as was specified in forms. The results showed that ventilation (fans and air inlets), roof and wall insulation in a poultry houses have represented about 55 percent of technological factors. The breeding units holding rank 1 and 2 enjoyed high degree of mechanization. As the level of mechanization was improved, the density of chickens per unit area was also increased; These units used more tunnel ventilation and large fans, and their ceiling were insulated using fiberglass, polystyrene, corrugated plastic and thickness of walls were also 35 cm. Mostly external heaters have been installed. Feeding and drinker systems have exerted no important effect on production. Slaughter weight and survival percent were not affected by the degree of mechanization but feed intake and feed conversion ratio decreased and production index increased (p<0.01) in the breeding units holding rank 1 and 2. In general in poultry house, ventilation system and ceiling and wall insulation improved energy efficiency and increased the efficiency of the system.
Sana Farhadi; Ali Akbar Masoudi; Rasoul Vaez Torshizi
Volume 17, Issue 1 , April 2015, , Pages 9-18
Abstract
The TLR4 gene structure as the main receptor for lipopolysaccharide recognition of Gram-negative bacteria was investigated in two strains of Iranian commercial Arian line and west Azerbaijan native chicks and its expression was studied in some major organs. Blood samples of the 120 birds were taken and ...
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The TLR4 gene structure as the main receptor for lipopolysaccharide recognition of Gram-negative bacteria was investigated in two strains of Iranian commercial Arian line and west Azerbaijan native chicks and its expression was studied in some major organs. Blood samples of the 120 birds were taken and total DNAs extracted. Then, the target gene was sequenced using four pairs of primers on four samples of each strain. The effects of amino acid changes on protein function were assessed by PANTHER software. To investigate the gene expression, total RNAs were extracted from liver, spleen, and lung tissues after slaughter of the birds. Gene expression was assessed by semi-quantitative RT-PCR. The amplified RNAs from tissues of liver, spleen and lungs. Then electrophoresis images were processed with the Image software and quantitative data analyzed by MINITAB. The results showed three new single polymorphisms (T1147C, C2246A and A1832G) in the sequence of TLR4 gene in the studied populations. The effects of variations on TLR4 protein structure indicated a deleterious effect of mutations on protein structure. The TLR4 gene expression in case of native and commercial strains did not show significant differences. Due to importance of the TLR4 in innate immunity and identification of some novel mutations affecting on protein structure, this gene could be a valuable candidate related to genetic resistance in poultry.